ABSTRACT
Resumo Fundamento Os glicocorticóides (GCs) são amplamente prescritos para o tratamento de numerosos distúrbios clínicos devido às suas propriedades anti-inflamatórias e imunomoduladoras, e um dos efeitos indesejáveis mais comuns desses medicamentos é a dislipidemia. Objetivo Avaliar o efeito da quercetina, um flavonoide derivado de plantas, no perfil lipídico de ratos tratados com glicocorticóides em altas doses. Métodos Um total de 32 ratos Sprague-Dawley foram distribuídos aleatoriamente entre quatro grupos (8 ratos por grupo) e tratados por 6 semanas com uma das seguintes opções : (i) solução salina normal; (ii) 40 mg/kg de succinato sódico de metilprednisolona (MP); (iii) MP + 50 mg/kg de quercetina; (iv) MP + 150 mg/kg de quercetina. O MP foi injetado por via subcutânea e a quercetina foi administrada por gavagem oral 3 dias por semana. No final do estudo, o perfil lipídico dos animais foi medido através de kits enzimáticos. Os dados foram analisados e a significância estatística foi estabelecida em p <0,05. Resultados Os níveis séricos médios de colesterol total (CT), triglicerídeos (TG) e LDL aumentaram drasticamente em animais tratados com GC em comparação com o grupo controle. Ambas as doses de quercetina (50 e 150 mg/kg) melhoraram o CT (43% e 45%), LDL (56% e 56%) e TG (46% e 55%, respectivamente). A razão Apo B/A1 diminuiu mais de 20% após a ingestão de Anti-Inflamatory Agents. Conclusões Esses dados sugerem que a ingestão de quercetina Quercetin; induzida por glicocorticóides. (Arq Bras Cardiol. 2020; 115(1):102-108)
Abstract Background Glucocorticoids (GCs) are widely prescribed for the treatment of numerous clinical disorders due to their anti-inflammatory and immune-modulatory properties and one of the most common untoward effects of these drugs is dyslipidemia. Objective To evaluate the effect of quercetin, a plant-derived flavonoid, on the lipid profile of high-dose glucocorticoid treated rats. Methods A total of 32 Sprague-Dawley rats, were randomly distributed among four groups (8 rats per group) and treated for 6 weeks with one of the following: (i) normal saline; (ii) 40 mg/kg methylprednisolone sodium succinate (MP); (iii) MP + 50 mg/kg quercetin; (iv) MP + 150 mg/kg quercetin. MP was injected subcutaneously, and quercetin was administered by oral gavage 3 days a week. At the end of the study, the animals' lipid profile was measured by enzymatic kits. Data were analyzed and statistical significance was set at p<0.05. Results The mean serum total cholesterol (TC), triglyceride (TG) and LDL levels were drastically increased in GC-treated animals compared with the control group. Both doses of quercetin (50 and 150 mg/kg) ameliorated TC (43% and 45%), LDL (56% and 56%) and TG (46% and 55% respectively). Apo B/A1 ratio decreased more than 20% following quercetin intake and the decline in TC/HDL, TG/HL, LDL/HDL ratios were significant. Conclusions These data suggest that quercetin intake with both doses of 50 and 150 mg/kg could be considered as a protective agent for glucocorticoid-induced dyslipidemia. (Arq Bras Cardiol. 2020; 115(1):102-108.)
Subject(s)
Animals , Rats , Quercetin/pharmacology , Glucocorticoids , Apolipoproteins , Triglycerides , Rats, Sprague-Dawley , LipidsABSTRACT
Aim: The aim of this study was evaluating the role of diet in induction and management of NonAlcoholic Fatty Liver Disease (NAFLD). Place and Duration of Study: The study was performed in Taleghani Hospital, Tehran, Iran for one year. Methodology: Dietary intakes of 24 patients with biopsy proven NAFLD referring to Taleghani Gastroenterology Clinic (Tehran, Iran) were analyzed by a detailed food frequency questionnaire. Dietary intervention including a proper dietary intake and advice on increasing individual activities was applied for them, and the effect of it was assessed after 6 months. Results: we did not find any significant differences between patients with NAFLD and healthy control population in the case of blood pressure, blood glucose, total cholesterol, and HDL cholesterol, but weight, height, BMI, waist, waist/hip ratio, and triglycerides, were significantly higher in patients than controls. The carbohydrate intake in patients was significantly more than controls while the protein consumption was significantly lower in patients than controls. We did not find any significant difference in the other dietary intakes. All anthropometric, biochemical, and histopathologic characteristics of the patients decreased after six months dietary intervention, however none of these differences were statistically significant. Conclusion: Our results indicate that dietary intake in patients with NAFLD is different from normal population, and a 6-month dietary and life-style consultation might result in improvement in clinical and para-clinical features of NAFLD.
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OBJECTIVES: In spite of growing evidence indicating the benefits of probiotics, the effects of different dietary oils on intestinal microflora and probiotics have not been elucidated. This study aimed to examine the effects of different dietary oils on intestinal microflora in an experimental model of colitis. METHODS: Eight-week mice were fed isocaloric diets varying only in fat composition for 4 weeks. The oils used were fish oil, canola oil, safflower oil, and chow diet containing beef tallow. Colitis was induced by intracolonic administration of acetic acid on day 21. The inflammation and fecal microflora and serum lipid profiles were evaluated 1 week after induction. RESULTS: Inflammation was highest in the chow diet group followed by safflower, canola, and fish oil fed groups, respectively. The number of fecal bacteroideceae was greater, whereas the number of fecal bifidobacteria was lower in mice fed beef tallow than the other ones. In addition, fish oil reduced the plasma level of triacylglycerole significantly. CONCLUSION: Polyunsaturated fatty acids (PUFAs) can affect intestinal microflora increasing the number of probiotics. PUFAs might be recommended in addition to probiotics for the prevention and/or maintenance treatment of colitis.
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OBJECTIVE: To determine the prevalence of gastrointestinal (GI) signs and symptoms in the general population in the northwestern area of Tabriz, Iran. METHODS: During May 2000, 4225 subjects were randomly chosen from a general population of approximately 230,000 individuals, and invited to a questionnaire interview and a clinical examination. RESULTS: Of the subjects invited, 3983 (94.2%) participated in the study. Among them, 569 (14.3%) reported one or more GI symptoms in the last 2 weeks, and 96 (2.4%) had GI signs. Abdominal pain (5.1%), constipation (3.6%), heartburn (3.0%), dyspepsia (2.9%), a mass prolapsing from the anus (2.1%), bloating (1.5%), and hematochezia (l%) were the most common GI symptoms. Abdominal tenderness on deep palpation was the commonest clinical sign (1.2%). CONCLUSION: The prevalence of GI symptoms in the population studied was lower than that reported from other populations.